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5 ht 3a receptor  (Novus Biologicals)


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    Structured Review

    Novus Biologicals 5 ht 3a receptor
    5 Ht 3a Receptor, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+ht+3a+receptor/5-HT3A+Antibody/pmc07385893-67-31-35
    Average 94 stars, based on 11 article reviews
    5 ht 3a receptor - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    SDS Page:

    Article Title: Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation
    Article Snippet: .. Protein samples were loaded and separated by 4–12% SDS-PAGE gel and transferred onto PVDF membrane, which were blocked in blocking buffer for 2 h and then incubated with primary antibodies for 5-HT 3A receptor (1:300, Novus Biologicals, NB10056351, Littleton, CO, USA), GluN1 receptor (1:1000, Abcam, Ab109182, Cambridge, MA, USA) and GAPDH (1:5000, Boster, BA1054, Wuhan, China) at 4 °C overnight. ..

    Membrane:

    Article Title: Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation
    Article Snippet: .. Protein samples were loaded and separated by 4–12% SDS-PAGE gel and transferred onto PVDF membrane, which were blocked in blocking buffer for 2 h and then incubated with primary antibodies for 5-HT 3A receptor (1:300, Novus Biologicals, NB10056351, Littleton, CO, USA), GluN1 receptor (1:1000, Abcam, Ab109182, Cambridge, MA, USA) and GAPDH (1:5000, Boster, BA1054, Wuhan, China) at 4 °C overnight. ..

    Blocking Assay:

    Article Title: Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation
    Article Snippet: .. Protein samples were loaded and separated by 4–12% SDS-PAGE gel and transferred onto PVDF membrane, which were blocked in blocking buffer for 2 h and then incubated with primary antibodies for 5-HT 3A receptor (1:300, Novus Biologicals, NB10056351, Littleton, CO, USA), GluN1 receptor (1:1000, Abcam, Ab109182, Cambridge, MA, USA) and GAPDH (1:5000, Boster, BA1054, Wuhan, China) at 4 °C overnight. ..

    Incubation:

    Article Title: Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation
    Article Snippet: .. Protein samples were loaded and separated by 4–12% SDS-PAGE gel and transferred onto PVDF membrane, which were blocked in blocking buffer for 2 h and then incubated with primary antibodies for 5-HT 3A receptor (1:300, Novus Biologicals, NB10056351, Littleton, CO, USA), GluN1 receptor (1:1000, Abcam, Ab109182, Cambridge, MA, USA) and GAPDH (1:5000, Boster, BA1054, Wuhan, China) at 4 °C overnight. ..

    Article Title: Spinal 5-HT 3 receptor contributes to somatic hyperalgesia induced by sub-chronic stress
    Article Snippet: .. The membranes were blocked in 5% nonfat milk for 2 h and then were incubated with primary antibody directed against 5-HT 3A receptor (1:300, Novus Biologicals, NB100-56351, Littleton, CO, USA) and against GAPDH (1:4000, Boster, BA2913) at 4°C overnight, respectively. .. The membranes were further incubated for 2 h in TBST buffer (10 mM Tris, 150 mM and 1 ml Tween-20 dissolved in 1 L distilled water with pH = 7.4–7.6) with goat anti-rabbit secondary antibody (Boster, BA1054) at 1:4000 dilution.



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    Image Search Results


    Journal: bioRxiv

    Article Title: Structural Determinants for Activity of the Antidepressant Vortioxetine at Human and Rodent 5-HT 3 receptors

    doi: 10.1101/2024.02.23.581731

    Figure Lengend Snippet:

    Article Snippet: The human wild-type 5-HT 3A receptor was also expressed in a developed stable, inducible cell line derived from HEK T-Rex 293 cell line (ATCC CRL-1573).

    Techniques: Membrane, Mutagenesis, Construct

    Journal: bioRxiv

    Article Title: Structural Determinants for Activity of the Antidepressant Vortioxetine at Human and Rodent 5-HT 3 receptors

    doi: 10.1101/2024.02.23.581731

    Figure Lengend Snippet:

    Article Snippet: The mouse wild-type 5-HT 3A receptor was expressed in a previously developed stable, inducible HEK-293 cell line from the parental T-Rex 293 cell line (ATCC CRL-1573) ( ; ; P olovinkin et al., 2018).

    Techniques: Membrane, Mutagenesis, Construct

    Effects of KAE on wild-type and mutant of  human 5-HT 3A  receptor.

    Journal: International Journal of Molecular Sciences

    Article Title: Hydroxy Pentacyclic Triterpene Acid, Kaempferol, Inhibits the Human 5-Hydroxytryptamine Type 3A Receptor Activity

    doi: 10.3390/ijms23010544

    Figure Lengend Snippet: Effects of KAE on wild-type and mutant of human 5-HT 3A receptor.

    Article Snippet: Human 5-HT 3A (GenBank accession No.: BC004453) DNA was obtained from OriGene (Rockville, MD, USA).

    Techniques: Mutagenesis